(1. 北京科技大学 土木与环境工程学院，北京 100083；
2. 中国科学院 过程工程研究所 生化工程国家重点实验室，北京 100190)
摘 要: 考察不同初始ρ(Mn2+)条件下，氧化亚铁硫杆菌(A.ferrooxidans)培养体系中Fe2+浓度的变化曲线和A.ferrooxidans菌的生长活性。结果表明:A.ferrooxidans菌对Mn2+具有一定的耐受能力；当培养基中ρ(Mn2+)≤1 g/L时，Mn2+对A.ferrooxidans菌生长活性影响很小；ρ(Mn2+)为5~15 g/L时，A.ferrooxidans菌生长开始受到抑制；ρ(Mn2+)=20 g/L时，A.ferrooxidans菌生长完全受到抑制。为了进一步描述Mn2+对其生长活性的影响，利用[Fe2+]/[Fe3+]的比值及A.ferrooxidans菌对Fe2+的得率系数对不同Mn2+浓度下细菌的比生长速率进行拟合，较好地描述Mn2+浓度对氧化亚铁硫杆菌生长的影响。
关键字: 氧化亚铁硫杆菌；生物浸锰；生长动力学；比生长速率
（1. School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,
Chinese Academy of Sciences, Beijing 100190, China）
Abstract:Acidithiobacillus ferrooxidans (A.ferroxidans) can be used for microbial leaching of manganese oxide, the impact of the different initial Mn2+ concentration on the activity of the growth of A.ferrooxidans was studied, and the bacterial growth and activity were obtained at different manganese ion concentrations. The results show that A.ferrooxidans is tolerant to Mn2+ some extent. The effect of Mn2+ on the growth and activity of A.ferrooxidans by Mn2+ is not bad when A.ferrooxidans are cultured in medium containing ρ(Mn2+)≤1 g/L. The growth and activity of A.ferrooxidans are obviously influenced by Mn2+ when ρ(Mn2+) is 5-15 g/L. When the concentration of Mn2+ is around 20 g/L, the growth and activity of A.ferrooxidans are completely inhibited. In orden to further describe the impact of Mn2+ on growth activity of A.ferrooxidans, [Fe2+]/[Fe3+] and the yield coefficient of Fe2+ on the specific growth rate of A.ferrooxidans in different concentrations of Mn2+ were fitted, the effect of Mn2+ concentration on the growth of A.ferrooxidans was described better.
Key words: Acidithiobacillus ferrooxidans; biological leaching manganese; growth kinetics; specific growth rate
