(1. 东北大学 资源与土木工程学院, 沈阳 110004;
2. 鞍山科技大学 土木与交通工程学院, 鞍山 114044)
摘 要: 研究了诺卡氏菌在不同影响因素条件下, 在黄铁矿和闪锌矿表面的选择性吸附。结果表明： 培养120 h后诺卡氏菌达到最大生长量； 诺卡氏菌在黄铁矿和闪锌矿表面发生明显的选择性吸附, 在黄铁矿表面的最大吸附率可达到96.99％, 而在闪锌矿表面的吸附率大都在20％以下； 诺卡氏菌在两种矿物表面于20 min内即可达到吸附平衡； pH值是影响诺卡氏菌在两种矿物表面发生选择性吸附的关键因素, pH在4～10之间时, 选择性吸附现象明显； 矿浆浓度超过6 g/L时有利于发生选择性吸附； 细胞悬浊液浓度、搅拌速度和温度对吸附效果影响不大, 均可产生选择性吸附。 扫描电镜检测结果表明, 诺卡氏菌细胞表面的菌丝是重要的吸附部位； 红外光谱分析结果表明, 诺卡氏菌表面含有—OH、 —NH2、 C==O、 P==O、 S==O等活性官能团, 它们在吸附过程中起重要作用。
关键字: 黄铁矿； 闪锌矿； 诺卡氏菌； 选择性吸附； 生物浮选
surface of pyrite and sphalerite
（1. School of Resources and Civil Engineering,
Northeastern University, Shenyang 110004, China;
2. School of Civil and Traffic Engineering,
Anshan University of Science and Technology, Anshan 114044, China）
Abstract: The selective adsorption of Nocardia on the surface of pyrite and sphalerite was studied under various conditions. The results show that the maximum growth quantity of Nocardia can be achieved after culturing 120 h and obvious selective adsorption of Nocardia on the surface of pyrite and sphalerite can be detected. The maximum adsorption rate on the surface of pyrite is 96.99%, whereas it is less than 20% in the case of sphalerite. Adsorption equilibrium can be attained within 20 min on the surfaces of the two minerals. pH value is the key factor influencing upon selective adsorption, and the difference of adsorption quantity on the surfaces of two minerals is apparent when pH is between 4 and 10. Slurry concentration over 6 g/L is favorable to selective adsorption. Nocardia cell concentration, stirring speed and temperature have no obviously effect on selective adsorption. SEM result indicates that the mycelium on the surface of ectoblast of Nocardia cell is the important attachable site. IR spectrum shows that the functional groups such as —OH, —NH2, C==O, P==O, S==O, etc make vital contribution on adsorption process.
Key words: pyrite； sphalerite； Nocardia； selective adsorption； bioflotation
