((1. 中南大学 资源加工与生物工程学院,长沙410083;2. 湖南科技大学 生命科学学院,湘潭410201))
摘 要: 以分离于江西某铜矿的嗜铁钩端螺旋菌(L. ferriphilum YSK)菌株为研究对象,报道不同Fe2+浓度对 L. ferriphilum生长活性的影响。结果表明,YSK菌株生长最适宜的Fe2+浓度约为0.1 mol/L。当初始Fe2+浓度为0.4 mol/L时,进入对数生长期前很明显需要一个较长的延迟期,表明该Fe2+浓度对细胞的生长产生较强的抑制作用;当初始Fe2+浓度为0.6 mol/L时,YSK菌株的生长完全受到抑制。尽管高浓度的Fe2+抑制细胞的生长,但受到抑制而不生长繁殖的YSK细胞仍然具有氧化Fe2+的能力。
关键字: L. ferriphilum YSK菌株;Fe2+;生长活性
((1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;2. School of Life Science, Hunan University of Science and Technology, Xiangtan 410201, China))
Abstract: The effect of different ferrous iron concentrations on the growth activity of L. ferriphilum was reported with L. ferriphilum YSK being developed objective of this research. The results show that YSK optimally grows at 0.1 mol/L of ferrous iron. In the culture initially containing 0.4 mol/L of ferrous iron, a long period of lag is observed, which is followed by exponential phase of microbial growth. However, in the culture initially containing 0.6 mol/L of ferrous iron, no evidence of bacterial growth is observed. Although ferrous iron is concentrations of 0.4 mol/L can prohibit the growth of cells completely, the non-growing population of L. ferriphilum YSK strain is able to oxidize ferrous iron.
Key words: L. ferriphilum YSK strain; ferrous iron; growth activity