(1. 中南大学 资源加工与生物工程学院，教育部生物冶金重点实验室，长沙 410083；
2. 东北大学 冶金学院，沈阳 110819)
摘 要: 为了获得耐砷能力较高的中度嗜热嗜酸浸矿菌Leptospirillum ferriphilum，采用连续转接驯化法进行耐砷驯化，并对菌株的驯化机制进行研究。结果表明：驯化后Leptospririllum ferriphilum菌的As(Ⅲ)和As(Ⅴ)耐受浓度分别提高到7.0 g/L和11.0g/L；As(Ⅴ)驯化菌株对砷黄铁矿的浸出作用明显比As(Ⅲ)驯化菌株强；砷驯化菌细胞官能团发生明显变化，多糖和蛋白质含量明显增多，细胞表面负电荷明显降低，以适应高砷的环境。同步辐射原位C-K边XANES谱图表明，驯化菌糖类和蛋白质的分布位置发生了变化；DFT理论计算表明，驯化菌更容易吸附到矿物表面上。
关键字: 砷；砷黄铁矿；黄铁矿；驯化
（1. Key Laboratory of Biometallurgy of Ministry of Education of China, School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China;
2. School of Metallurgy, Northeastern Univwrsity, Shenyang 110819, China）
Abstract:In order to obtain high arsenic-tolerant ability of Leptospirillum ferriphilum, continuous transfer domestication was adopted, and the acclimation mechanism was studied. The results show that, after domestication, the tolerant abilities to As(Ⅲ) and As(Ⅴ) of L. ferriphilum increase to 7.0 g/L and 11.0 g/L, respectively. The leaching effect of the bacteria acclimated by As(Ⅴ) is significantly higher than that by As(Ⅲ) in the leaching of arsenopyrite. After being acclimated, the contents of polysaccharide and protein of cells significantly increase, while the negative charge on the surface of cells significantly decreases, so as to adapt to the high arsenic environment. The in-situ C-K-edge XANES results show a change in the distribution of polysaccharide and protein. The DFT calculation shows that the adapted bacterial cells are easier to be adsorbed on the mineral surface.
Key words: arsenic; arsenopyrite; pyrite; domestication
