(1. 中南大学 湘雅三医院，长沙 410013；
2. 中南大学 粉末冶金国家重点实验室，长沙 410083；
3. 中南大学 医用材料与器械研究中心，长沙 410013)
摘 要: 以硝酸钙、磷酸氢二氨等为反应原料，采用水热合成法制备羟基磷灰石(HAP)纳米颗粒，对该HAP颗粒以及添加精氨酸(Arg)或掺杂少量稀土离子铽(Tb)/铕(Eu)对HAP颗粒的形貌修饰等进行研究，采用透视电镜(TEM)、X射线衍射仪(XRD)、傅立叶红外光谱仪(FTIR)等，对制备样品的结晶性、粒度、分散性等进行分析测试。结果表明:精氨酸表面修饰改变HAP纳米颗粒的表面Zeta电位，从而在一定程度上抑制HAP的生长速率；少量稀土离子Eu/Tb掺杂并不影响HAP/Arg纳米颗粒产物的结构，均为单一的HAP物相，所合成产物的粒径也为纳米级。经稀土金属铽/铕掺杂的精氨酸表面修饰的HAP纳米颗粒可作为基因转染载体。
关键字: 羟基磷灰石；水热合成；精氨酸修饰；稀土掺杂
（1. The Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
3. Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China）
Abstract:Nano-hydroxyapatite(HAP) was prepared by a hydrothermal method with calcium nitrate, diammonium hydrogen phosphate as raw material, and to study its characteristics for morphology modification of arginine-functionalized and doped with rare earth, such as Eu3+ or Tb3+. The crystallization, grain size and dispersibility of the sample HAP were analyzed and discussed. The results show that the surface Zeta potential of arginine-functionalized HAP is changed, and the growth rate of HAP is inhibited to a certain extent during the synthesis. The structure of HAP/Arg is not affected during the synthesis doped by a small amount of rare earth ions, such as Eu3+ or Tb3+. All these samples have single phase of HAP with good dispersibility. The synthesized HAP is also nano-sized level. Nano-hydroxyapatite with arginine functionalized and rare earth doped, such as Eu3+ or Tb3+, is suitable for the application of gene delivery as a gene carrier.
Key words: hydroxyapatite; hydrothermal synthesis; arginine modification; rare-earth doping
