(1. 中南大学资源加工与生物工程学院，长沙410083；
2. 中南大学教育部生物冶金重点实验室，长沙410083)
摘 要: 与传统微生物生态学分析方法相比，近几年高通量测序以速度快、通量高、低成本等特征在微生物多样性检测方面得到了充分应用，并采用此方法研究温度对微生物浸出赞比亚一种低品位铜矿的效率和群落生态的影响。结果表明：在30℃和45℃浸出条件下，铜浸出率分别为64.2%和69.4%，45℃条件下获得了较高铜浸出率。采用高通量Illumina Miseq测序技术分析30℃和45℃浸出条件下微生物群落结构及其动态变化，当浸出温度为30℃，Acidithiobacillus caldus和Acidiphilium sp.是主要浸矿菌；当浸出温度为45℃时，Sulfobacillus sp.和Acidithiobacillus caldus是优势种群。
关键字: 铜矿；温度；生物浸出；群落动态；Illumina Miseq测序
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China）
Abstract:The main features of high-throughput sequencing are high speed, high-throughput and low cost compared with the traditional microbial ecology analysis method. In recent years, the high-throughput sequencing technology gets full uses in the investigation of microbial diversity. The effects of temperature on bioleaching of low grade copper ore and the dynamics of microbial community from Zambia were studied. The results show that, at30℃ and 45℃, the copper leachingratesare 64.2% and 69.4%, respectively. The rise of temperature results in higher copper leaching rate at 45℃. The shift of microbial community at 30℃ and 45℃ during bioleaching process was analyzed by Illumina Miseq sequencing platform. The results show that Acidithiobacillus caldus and Acidiphilium sp. are the main microorganisms at 30℃. While at 45℃, Sulfobacillus sp. and Acidithiobacillus caldus dominate in the microbial community.
Key words: copper ore; temperature; bioleaching; microbial community dynamics; Illumina Miseq sequencing
