中国有色金属学报(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 25 No. 9 September 2015 |
（1. Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
3. School of Materials Science and Engineering, Central South University, Changsha 410083, China）
Abstract:Porous titanium with porosity of 60% was prepared by metal injection molding (MIM), and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method. The effects of porous titanium coated with insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) gelatin microspheres on the function of MG63 cells were evaluated in vitro. The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity. The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg, respectively. The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg, respectively. The joint application of IGF-1 and TGF-β1 group, which promote adhesion, proliferation and differentiation of MG63 cells, is superior to a single application group.
Key words: porous titanium; gelatin microsphere; insulin-like growth factor-1; transforming growth factor-β1; MG63 cell
