中国有色金属学报(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 24 No. 12 December 2014 |
（1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;
2. Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education,
Hunan University, Changsha 410082, China）
Abstract:The protective role of exogenously supplied selenium (Se6+) on Bechmeria nivea (L.) Gaud (Ramie) subjected to cadmium (Cd) stress was studied in vitro, and the mechanism was discussed by investigating plant growth, malondialdehyde (MDA), activity of antioxidative enzymes and DNA methylation pattern. Plants grown in hydroponic culture were supplied with spraying Se (selenate, 1.2 μmol/L) and cadmium (Cd(NO3)2, 0, 3, 6 and 9 mg/L), individually or simultaneously. At low Se spraying levels, SOD activity was increased by 35.34%, 43.18%, 3.63% under 3, 6 or 9 mg/L cadmium contents, POD was increased by 12.45%, 14.14%, 3.27%, and the level of DNA methylation was decreased by 10.70%, 18.18% and 15.59%, respectively. The results confirmed that spraying low Se on ramie leaves could enhance the activity of SOD and POD, and regulate DNA methylation in ramie leaves.
Key words: ramie; Se; Cd; DNA methylation; antioxidative enzyme; phytoremediation
