(1. 南华大学 核资源工程学院，衡阳 421001；
2. 南华大学 铀矿冶生物技术国防重点学科实验室，衡阳 421001；
3. 中南大学 资源加工与生物工程学院，长沙 410083)
摘 要: 为了探明混合浸矿微生物的耐氟特性及其群落动态，构建5株浸矿细菌(Acidithiobacillus ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01, Acidithiobacills caldus S1)的共培养体系。结果表明：氟胁迫在一定程度上抑制了该共培养体系的生长繁殖，对于高浓度的氟胁迫，共培养体系也保持较为平稳的生长延滞状态；氟胁迫对共培养体系硫氧化活性的影响并不明显。采用实时荧光定量PCR技术对其培养体系的群落动态进行分析。结果表明，不论是氟胁迫下还是无氟胁迫下，L. ferriphilum YSK和A. caldus S1，都是群落中的优势种群；而A. ferrooxidans ATCC 23270、S. thermosulfidooxidans ST和A. thiooxidans A01为劣势种群。共培养体系中受氟胁迫影响最大的是S. thermosulfidooxidans ST，菌体密度急剧下降；其次是A. caldus S1、A. thiooxidans A01和A. ferrooxidans ATCC 23270，氟胁迫在一定程度上也抑制了对它们的生长；而L. ferriphilum YSK在氟胁迫下的生长非常稳定。
关键字: 浸矿微生物；共培养体系；氟耐受性；群落动态
（1. School of Nuclear Resources Engineering, University of South China, Hengyang 421001, China;
2. Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,
University of South China, Hengyang 421001, China;
3. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:In order to explore the fluoride tolerance and the community dynamics of mixed bioleaching microorganisms, a co-culture including five typical bioleaching strains (Acidithiobacillus ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01, Acidithiobacills caldus S1) was constructed. The results show that the growth of the co-culture is inhibited by fluoride stress, and the co-culture keeps the stable cell density at high fluoride stress. The sulfur activity of the co-culture is not affected by fluoride stress. Microbial community dynamics of the co-culture was analyzed by real-time fluorescent quantitative PCR technology. The results show that L. ferriphilum YSK and A. caldus S1 are always the dominant species at fluoride stress or non-stress, while the minor is A. ferrooxidans ATCC 23270, S. thermosulfidooxidans ST and A. thiooxidans A01. Besides, S. thermosulfidooxidans ST is inhibited most obviously in the co-culture at fluoride stress, secondly A. caldus S1, A. thiooxidans A01 and A. ferrooxidans ATCC 23270, while the L. ferriphilum YSK still maintains a very stable growth.
Key words: bioleaching microorganisms; co-culture; fluoride tolerance; community dynamics
