中国有色金属学报(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 23 No. 9 September 2013 |
（1. School of Metallurgy and Environment, Central South University, Changsha 410083, China;
2. Hunan Key Laboratory of Green Packaging and Biological Nanotechnology,
Hunan University of Technology, Zhuzhou 412007, China;
3. School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:The taxonomy characteriazation and cadmium (Cd) biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers (ITS) region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.
Key words: taxonomy characterization; cadmium biosorption; Paecilomyces lilacinu; Fourier transform infrared spectroscopy
