(1. 中南大学 冶金科学与工程学院，长沙 410017；
2. 中机国际工程设计研究院有限责任公司，长沙 410007)
摘 要: 以pannonibacter phragmitetus T1菌作为生物吸附剂，研究细菌代谢活性、培养时间、溶液pH值、菌体用量、温度、吸附时间、初始金属浓度等因素对其吸附重金属Pb2+的影响，并进一步研究吸附机理。结果表明，培养12 h的灭活T1菌体在Pb2+浓度为150 mg/L、pH值为6、T1菌添加量为0.5 g/L、温度为30 ℃的条件下，对Pb2+的最大吸附量为68.35 mg/g；灭活T1菌对重金属Pb2+的吸附过程较快，90 min即达到吸附平衡，Pb2+的吸附符合Pseudo-second order模型和Langmuir等温吸附模型。结合红外光谱分析，灭活pannonibacter phragmitetus T1菌对Pb2+是表面吸附，羟基和酰胺基是与Pb2+络合反应的主要基团。
关键字: pannonibacter phragmitetus T1菌；铅离子；吸附条件；吸附机理
（1. School of Metallurgical Science and Engineering, Central South University, Changsha 410017, China;
2. China Machinery International Engineering Design & Research Institute Co., Ltd., Changsha 410007, China）
Abstract:The pannonibacter phragmitetus T1 was used as bio-sorbent, the effects of the factors such as the metabolic activity of bacteria, culture time, pH value, biomass concentration, temperature, adsorption time and initial concentration of metal ions on the biosorption of Pb2+ were determined, and the adsorption mechanism was further discussed. The results show that the maximum adsorption capacity of T1 for Pb2+ reaches 68.35 mg/g under the optimal conditions as the Pb2+ concentration of 150 mg/L, pH value of 6, the nonliving bacteria cultured after 12 h with the biomass of 0.5 g/L, and temperature of 30 ℃. The kinetic study of the adsorption shows that the adsorption process of Pb2+ is rapid, and finishes in 90 min. The adsorption process is well accorded with the Pseudo-second order model and Langmuir model. The analysis of the FTIR spectrum indicates that the hydroxyl and amide are the main reactive groups on the surface adsorption.
Key words: pannonibacter phragmitetus T1; Pb2+; biosorption condition; biosorption mechanism
