(1. 湖南有色金属研究院，长沙 410015；2. 中南大学 冶金科学与工程学院，长沙 410083)
摘 要: 通过对培养基的优化，提出并研究直接添加培养基激活土著微生物的活性来进行铬渣堆场污染土壤的原位微生物修复新方法，并探讨土壤环境对土著微生物修复Cr(Ⅵ)效果的影响。研究表明：在每千克土壤中碳源葡萄糖投加量为5 g、氮源化合物A投加量为5 g、温度为30 ℃、土与液质量比为1:1的情况下，土著微生物可被迅速激活，在第4天时能基本去除土壤的中水溶性Cr(Ⅵ)；培养基初始pH值和不添加无机盐氯化钠不影响其修复效果。
关键字: 铬渣堆场；铬污染；土著微生物；原位修复
（1. Hunan Research Institute of Nonferrous Metals, Changsha 410015, China;
2. School of Metallurgical Science and Engineering, Central South University, Changsha 410083, China）
Abstract:Based on the optimization of culture medium composition, the growth conditions and ability of Cr(Ⅵ) reduction, the in-situ remediation of Cr-contaminated soil can be achieved by adding culture medium in soils to stimulate the activity of indigenous microorganism. The results show that the optimal conditions for the Cr(Ⅵ) reduction by indigenous microorganism are 5 g glucose and 5 g nitrogenous compounds A per kilogram soil at 30 ℃ and the mass ratio of soil to water is 1:1. Under the optimal condition, the water soluble Cr(Ⅵ) in the soil contaminated by chromium-containing slag heap is completely removed in the fourth day. The initial pH value of culture medium do not affect Cr(Ⅵ) reduction. The inorganic salt does not need to be added into the culture medium when the in-situ remediation of Cr(Ⅵ) is carried out by the indigenous microorganism.
Key words: chromium-containing slag heap; Cr-contamination; indigenous microorganism; in-situ remediation
