(1. 中南大学 湘雅三医院，长沙 410013；
2. 中南大学 粉末冶金国家重点实验室，长沙 410083)
摘 要: 采用碱热处理和仿生沉积HA对注射成形多孔钛进行表面处理，研究改性后多孔钛的细胞毒性及其成骨细胞黏附、增殖和分化的影响。结果表明：改性后多孔钛无细胞毒性，不同孔隙度多孔钛的细胞黏附形貌不同，复合培养7 d后，5%孔隙度组细胞平铺于材料表面，30%孔隙度组细胞多呈长梭形，60%孔隙度组MG63细胞伪足以锚状牢固附着于孔壁的颗粒之上，形成细胞桥；成骨细胞在高孔隙度组表面细胞的增殖数高于低孔隙度组表面细胞的；复合培养7 d与14 d，5%孔隙度组与30%孔隙度组的上清液中碱性磷酸酶(Alkaline phosphatase, ALP)含量无明显差别(p＜0.05)，而60%孔隙度组的ALP含量高于低孔隙度组的。
关键字: 生物材料；医用钛合金；多孔钛；孔隙度；黏附；增殖；碱性磷酸酶
（1. Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China）
Abstract:The metal injection molding (MIM) method was used to fabricate porous Ti implants, the surface modification of the porous titanium was performed using alkali-heat treatment and biomimetic hydroxyapatite (HA) deposition. The influence of modified porous titanium on the cell toxicity, adhesion, proliferation and differentiation of the osteoblast cells were investigated. The results show that the modified porous titanium is nontoxic to cells. The morphologies of the cells vary with change of porosity of porous titanium. After cultured 7 d, MG63 is flat on the surface of 5% porosity titanium implant and extends to amphi-edge of the pore on the surface of 30% porosity titanium implant. The titanium implant with porosity of 60% can be clearly noticed that the pseudopodias adhere deeply to the hole wall and form the cell bridges, and the cells can grow into the pores. The number of the cell proliferation in high porosity groups is higher than that in low group. The alkaline phosphatase(ALP)content is influenced by the porosity of porous titanium, there is no significant difference between 5% and 30% porosity group (p＜0.05), the ALP content of 60% porosity group is higher than that of low porosity group.
Key words: biomaterial; medical titanium alloy; porous titanium; porosity; adhesion; proliferation; alkaline phosphatase
