(中南大学 资源加工与生物工程学院 生物冶金教育部重点实验室，长沙 410083)
摘 要: 以嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans, ATCC23270)为对象，研究NO3−、SO42−、Cl−和PO43−对Acidithiobacillus ferrooxidans生长和硫氧化活性的影响。结果表明：不同阴离子对Acidithiobacillus ferrooxidans硫氧化表观活性的影响效应不同，其影响由大到小的顺序依次为Cl−，NO3−，SO42−和PO43−；在高浓度PO43−的胁迫(300 mmol/L)下，细菌所表达蛋白质的总数量减少，但同时表达一些特异性蛋白质，这说明细菌可通过抑制一些在正常条件下表达的蛋白质，同时表达一些新的蛋白质来应对无机离子的胁迫效应。
关键字: 嗜酸氧化亚铁硫杆菌；阴离子；胁迫效应
Acidithiobacillus ferrooxidans
（Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering,
Central South University, Changsha 410083, China）
Abstract:The effects of NO3−, SO42−, Cl− and PO43− on the growth of Acidithiobacillus ferrooxidans and sulfur oxidation activity were investigated. The results show that the anions have different effects on the sulfur oxidation activity, and the inhibition effect sequence from great to little is Cl−, NO3−, SO42−, PO43−. The total proteins of Acidithiobacillus ferrooxidans grown under PO43− threatening condition (300 mmol/L) are down-regulated, but specific proteins are observed, when compared with normal condition. These specific proteins may take part in the response to the stress effect of anions.
Key words: Acidithiobacillus ferrooxidans; anion; stress effect
