(中南工业大学 冶金科学与工程系, 长沙 410083)
摘 要: 基于Monod方程,推导并建立了氧化亚铁硫杆菌TD-10在含亚铁培养基中的生长响应模型 。研究了以亚铁离子的氧化来表征氧化亚铁硫杆菌的生长过程; 利用该模型, 通过计算机拟合, 确 定了模型中细菌生长动力学参数,并用该模型预示了最大比生长速率μm,营养 物饱和常数 K 及细菌的初始浓度与得率系数的比值ρ0b/Y 变化时对细菌 生长的影响。考察了不同二价铁离子初始浓度、 温度和接种量时亚铁离子浓度的变化, 从 而确定了各实验条件下的μm,K 及ρ0b/Y 值。
关键字: 氧化亚铁硫杆菌; 生长响应模型; 动力学参数
(Department of Metallurgical Science and Engineering, Central South University of Technology, Changsha 410083, P.R.China)
Abstract:Based on Monod formula, a responsive mathem atical model for a cer tain kind of Thiobacillus ferrooxidans named TD-10 growing in the substrate con taining ferrous ion was built up. With computer simulating variation of f errous ion concentration during the bacteria growth, this model can be used to determine the kinetic parameters of bacteria growth, and the influence of these parameters on bacteria growth can be predicted. Under th e varie d experimental conditions of initial ferrous ion concentration, temperature and inocu lation amount, the ferrous ion concentration was examined, and the experimenta l data were simulated by computer to determine μm, K and ρ0b/Y. Furthermore, the influence of experimental conditions on these parameters was discussed.
Key words: Thiobacillus ferrooxidans; oxidation of f errous ions; bacteria growth model; kinetic parameters