(1. 中国科学院 过程工程研究所 生化工程国家重点实验室，北京 100080；
2. 北京科技大学 土木与环境工程学院，北京 100083)
摘 要: 以微生物燃料电池为研究工具，研究Rhodoferax ferrireducens(R.f )异化还原钒(Ⅴ)的过程。在对R.f进行NaVO3耐性驯化实验的基础上，考查NaVO3废水浓度、pH值和温度对R.f还原钒(Ⅴ)的影响。结果表明：适量钒元素对微生物生长具有促进作用，微生物的生长趋势及周期与钒(Ⅴ)的还原趋势及周期基本相同；初期由于钒化合物的生物效应促进葡萄糖消耗量增加，葡萄糖氧化分解过程产生小分子有机酸，使溶液pH降低，但钒(Ⅴ)还原过程需要H+的参与，且R.f异化作用也消耗部分小分子有机酸，后期溶液pH升高。经优化其培养条件为：温度30 ℃、搅拌速度150 r/min、pH为7.5时，在NaVO3含量为300 mg/L的钒废水中，R.f对钒(Ⅴ)的还原率为76%，细菌总数达到1.1×109 cfu/mL。
关键字: 钒冶金废水；Rhodoferax ferrireducens；微生物燃料电池；异化还原
（1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,
Chinese Academy of Sciences, Beijing 100080, China;
2. School of Civil and Environmental Engineering, University of Science and Technology Beijing,
Beijing 100083, China）
Abstract:The process of Rhodoferax ferrireducens (R.f ) dissimilatory reduction of V5+ in waste water was studied by the means of microbial fuel cell (MFC). Based on the inoculation test of tolerance to NaVO3 of R.f, the effects of NaVO3 concentration in the wastewater, pH and temperature on R.f reducing V5+ were investigated. The results show that adequate vanadium can promote the growth of microbes, and the tendency and cycle of R.f growth are essentially the same as that of V5+ reducing. In beginning period, the bioeffect of vanadium compound increases the consumption of glucose, and the glucose is oxidized to the small molecule organic acids, which leads to pH value fall. But H+ should be engaged in V5+ reduction, and the dissimilatory reduction of R.f will also consume some small molecule organic acids, which makes pH increase later. The optimized culture conditions are as follows: temperature 30 ℃, stirring speed 150 r/min, pH 7.5, NaVO3 concentration in the vanadium wastewater 300 mg/L. The reduction rate of V5+ by R.f is 76%, and the total R.f count reaches 1.1×109 cfu/mL.
Key words: vanadium metallurgical waste water; Rhodoferax ferrireducens; microbial fuel cell; dissimilatory reduction
