Transactions of Nonferrous Metals Society of China The Chinese Journal of Nonferrous Metals

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中国有色金属学报

ZHONGGUO YOUSEJINSHU XUEBAO

第12卷    第1期    总第46期    2002年2月

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文章编号:1004-0609(2002)01-0014-06
金属毒性研究(Ⅱ)
阮建明1,2,M.H.Grant2,黄伯云1

(1. 中南大学 粉末冶金国家重点实验室,长沙 410083;
2. Bioengineering Unit,Strathclyde University,
Glasgow G4 ONW, UK
)

摘 要: 利用细胞生物学技术从定量和定性两个方面探讨了Cr(Ⅲ),Cr(Ⅵ),Ni,Al,Ag和V金属离子对生命组织的毒性作用。研究中发现,Cr(Ⅵ)对细胞DNA,RNA合成限 制最为显著,Ni和V金属离子在同等水平上妨碍RNA合成,随着离子浓度增加,Cr(Ⅲ)离子对细胞DNA及RNA限制增大。Al离子对RNA合成限制大于对DNA合成。Ag离子对细胞DNA,RNA合成限制作用相同。细胞谷胱甘肽(GSH)还原能力强,是细胞最重要的解毒物质,也是细胞中防御毒性物质最关 键的物质。细胞GSH还原能力定量分析表明:微量的Cr(Ⅵ)即可导致细胞GSH下降。金属Ni离子破坏细胞骨架,使细胞内信息传递受阻,亦表现较强毒性。

 

关键字: 金属毒性;DNA和GSH;细胞

Approach of metal cytotoxicity(Ⅱ)
RUAN Jian-ming12, M.H.Grant2, HUANG Bai-yun1

1.State Key Laboratory for Powder Metallurgy,
Central South University,Changsha 410083,China;
2. Bioengineering Unit,Strathclyde University,
Glasgow G4 ONW,UK

Abstract:By use of cellular biological techniq ues,the poison mechanism of metal ions (Cr(Ⅵ),Ni,V,Cr(Ⅲ),Ag,and Al) on livi ng tissue was both quantitatively and qualitatively approached. The re sults show that Cr(Ⅵ) has a notable action on cellular both DNA and RNA synth etic functions. With increasing concentrat ion,the limit action of Cr(Ⅲ),on DNA,RNA synthetic abilities increases. Di fferent to oth er five metal ions,the limit of Al ion on RNA is greater than that of Al ion on DNA.The limitation of V,Ag ions on DNA is same to that of V,Ag ions on RNA. Glutathione is most important of detoxification in living cells. It is also the most important defensive. Trace Cr(Ⅵ),in culture medium results in the decreasing of GSH in living tissue. Similar to that,a little higher Ni ion concentration seriously reduces the GSH content in living tissue. It is suggested that Cr(Ⅵ),Ni should destroy and/or disturb the orientation of the microtubulin in cell skeleton. For other four metalions,together with increasing the concentration in culture medium, the osmotic pressure increases. Consequently,more metal ions entered cell membrane,more GSH were lost in cell.

 

Key words: metal cytotoxicity; DNA and GSH; cell

ISSN 1004-0609
CN 43-1238/TG
CODEN: ZYJXFK

ISSN 1003-6326
CN 43-1239/TG
CODEN: TNMCEW

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