(兰州大学 生命科学学院， 兰州 730000)
摘 要: 以氧化亚铁硫杆菌野生菌（wide T.f）和驯化菌（adapted T.f）为供试菌株， 通过比较野生菌与驯化菌对Cu2+的吸收与运输特性，研究了氧化亚铁硫杆菌驯化菌株的抗铜机理。 结果表明： Cu2+对野生菌Fe2+的氧化活性影响较大， 当Cu2+浓度高于10 g/L时，细菌Fe2+氧化活性逐渐降低直至完全抑制； 而经驯化培养的T.f菌， 对重金属铜则具有较高的抗性， 其Fe2+氧化活性也较高， Fe2+氧化速率加快； 在不同Cu2+浓度下，驯化菌细胞中的Cu2+含量总低于野生菌的， 经驯化培养的氧化亚铁硫杆菌与野生菌相比， 具有更高的促进体内
Cu2+排出的能力， 该排出系统与ATPase的活性有关。
关键字: 氧化亚铁硫杆菌； 铜离子； 运输特性； 氧化活性
in adapted Thiobacillus ferrooxidans
（ School of Life Science, Lanzhou University, Lanzhou 730000, China）
Abstract: Copper-resistance mechanisms of the adapted Thiobacillus ferrooxidans were studied in this thesis by comparing the uptake and transport characterization with Cu2+ in the wide T.f and the adapted T.f. The results show that wide T.f is largely affected by Cu2+ when the content of Cu2+ is bigger than
10 g/L, the active oxidation of Fe2+ reduces gradually till completely restraining. The adapted T.f is quite resistant to the heavy metal copper, exhibiting in the active oxidation of Fe2+ and its speeding oxidation rate. Further, the accumulation of Cu2+ in the cell of the adapted T.f is always lower than the wide T.f. Compared with the wide T.f, the adapted T.f possesses higher power in exporting Cu2+ and the efflux system is related to the activity of ATPase.
Key words: Thiobacillus ferrooxidans; Cu2+ ion; transportation characterization; oxidation activity
