(1.中南大学 资源加工与生物工程学院，长沙 410083；2.中南大学 生物冶金教育部重点实验室，长沙 410083)
摘 要: 从云南腾冲酸性热泉分离出一株嗜酸热异养菌TC-2。该菌呈长杆状，细胞大小为(2.0~5.0) μm×(0.3~0.5) μm，革兰氏染色反应为阴性，能形成芽孢。该菌生长温度范围为45~70 ℃，最适生长温度为55 ℃，适宜生长的pH值为1.5~4.0，最适宜生长的pH值为2.5，该菌能利用多种有机物生长。16S rDNA序列分析显示其与Alicyclobacillus sendaiensis的16S rDNA相似性大于99%。该菌自身没有浸矿作用，但对Acidianus manzaensis、Sulfolobus metallicus和Metallosphaera sedula等高温浸矿菌的浸矿有一定促进作用。
关键字: 嗜酸热异养菌(Alicyclobacillus sendaiensis)；分离；鉴定；黄铜矿；生物浸出
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China）
Abstract:A heterotrophic acidothermophilic bacterium TC-2 was isolated from an acid spring sample collected in Tengchong, Yunnan, China. The gram-negative-staining cells of the strain were straight long rods, 2.0−5.0 µm in length and 0.3−0.5 µm in diameter. The temperature range for growth was from 45 ℃ to 70 ℃ with an optimum temperature of 55 ℃. The pH range for growth at 55 ℃ was from 1.5 to 4.0 with an optimum at 2.5. This bacterium can use a wide variety of organic compounds to support fast growth, but can not utilize chalcopyrite as energy source. 16s rRNA gene Analysis reveal that this strain closely relates to Alicyclobacillus sendaiensis, with over 99% sequence similarity between each other. Co-bioleaching of chalcopyrite with Acidianus manzaensis，Sulfolobus metallicus and Metallosphaera sedula shows that the strain TC-2 can improve leaching rates of the sulphide minerals.
Key words: heterotrophic acidothermophile(Alicyclobacillus sendaiensis); isolation; characterization; bioleaching; chalcopyrite
