(中南大学 资源加工与生物工程学院，长沙 410083)
摘 要: 采用连续稀释法分别从江西德兴铜矿、湖北大冶铜矿和广西大厂这3处酸性矿坑水样品中分离得到三株嗜酸性短杆状细菌，其形态及生理特征与嗜酸性氧化亚铁硫杆菌相似，16S rRNA系统学分析进一步证实这种细菌属于硫杆菌属的嗜酸性氧化亚铁硫杆菌。研究表明：阴离子对嗜酸氧化亚铁硫杆菌的Fe2+氧化能力具有一定影响，其影响顺序由大到小依次为：NO3＞Cl−＞SO4；不同菌株对阴离子的耐受能力存在明显的差异。
关键字: 嗜酸氧化亚铁硫杆菌； ；Cl−；
（School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:By successive diluting method three acidicbacillus strains were isolated from the samples of acid mine drainage taken from Dexing Copper Mine in Jiangxi province, Daye Copper Mine in Hubei province and Dachang Copper Mine in Guangxi province. The three Acidithiobacillus strains are similar to Acidithiobacillus ferrooxidans in shapes and biological characters. 16S rRNA gene sequence analysis further confirms that the three bacteria strains are Acidithiobacillus ferrooxidans. The results show that anions obviously impact the oxidation capacity of ferrous ions of these bacteria with an effect order as: ＞Cl−＞ . And their tolerances to anions are obviously different from each other.
Key words: Acidithiobacillus ferrooxidans; ; Cl−;
