Transactions of Nonferrous Metals Society of China The Chinese Journal of Nonferrous Metals

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Transactions of Nonferrous Metals Society of China

Vol. 20    No. 3    March 2010

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Isolation, characterization and extraction of mer gene of Hg2+ resisting strain D2
ZENG Xiao-xi(曾晓希)1, 2, TAGN Jian-xin(汤建新)2, JIANG Pei(蒋 佩)2,
LIU Hong-wei(刘宏伟)1, DAI Zhi-min(戴志敏)1, LIU Xue-duan(刘学端)

1. School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Hunan Key Laboratory of Green Packaging and Biological Nanotechnology,
Hunan University of Technology, Zhuzhou 412007, China

Abstract:Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence, growth, minimal inhibitory concentrations (MICs) of metals, antibiotic susceptibility and mer gene were conducted. The strain D2 can grow in the medium containing 60 mg/L mercury ion. It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa, and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance. The mer RT gene sequence was amplified from chromosome. Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.


Key words: isolation; mercury-resistant strain; Pseudomonas aeruginosa; mer RT

ISSN 1004-0609
CN 43-1238/TG

ISSN 1003-6326
CN 43-1239/TG

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