Transactions of Nonferrous Metals Society of China The Chinese Journal of Nonferrous Metals

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中国有色金属学报(英文版)

Transactions of Nonferrous Metals Society of China

Vol. 20    No. 2    February 2010

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Bioleaching of chalcopyrite by UV-induced mutagenized Acidiphilium cryptum and Acidithiobacillus ferrooxidans
XU Ai-ling(徐爱玲), XIA Jin-lan(夏金兰), ZHANG Shuai(张 帅),
YANG Yu(杨 宇), NIE Zhen-yuan(聂珍媛), QIU Guan-zhou(邱冠周)

Key Laboratory of Biometallurgy of Ministry of Education of China,
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

Abstract:The original strains Acidithiobacillus ferrooxidans GF and Acidiphilium cryptum DX1-1 were isolated from the drainage of some caves riched in chalcopyrite in Dexing Mine in Jiangxi Province of China. The optimum temperature and pH for growth were 30 ℃ and 3.5 for Ac. cryptum DX1-1, and 30 ℃ and 2.0 for At. ferrooxidans GF, respectively. For Ac. cryptum DX1-1, the optimum UV radiating time was 60 s and the positive mutation rate was 22.5%. The growth curves show that Ac. cryptum after mutagenesis reached stationary phase within 60 h, which was 20 h earlier than the original strain. For At. ferrooxidans GF, the optimum mutation time was 60 s and the positive mutation rate was 35%. The most active UV-mutated strain At. ferrooxidans GF oxidized all the ferrous after 48 h. The bioleaching experiments showed that bioleaching with the mixture of UV-mutated strains of At. ferrooxidans GF and Ac. cryptum DX1-1(1׃1) could extract 3.01 g/L of copper after 30 d, while the extracted copper was 2.63 g/L with the mixture of the original strains before UV-mutation. At the end of the bioleaching experiments, the proportion of the cell density in the cultures of Ac. cryptum DX1-1 and At. ferrooxidans GF was approximately 1׃5.

 

Key words: Acidiphilium cryptum; Acidithiobacillus ferrooxidans; UV-induced mutagenesis; bioleaching; chalcopyrite

ISSN 1004-0609
CN 43-1238/TG
CODEN: ZYJXFK

ISSN 1003-6326
CN 43-1239/TG
CODEN: TNMCEW

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