中国有色金属学报(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 18 No. 6 December 2008 |
autotrophicbioleaching bacteria
（1. Key Laboratory of Biometallurgy of Ministry of Education,
School of Minerals Processing and Bioengineering, Central South University,
Changsha 410083, China;
2. China Center for Type Culture Collection, College of Life Science, Wuhan University,
Wuhan 430072, China）
Abstract:Three kinds of autotrophic bioleaching bacteria strains, including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans), mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans (A. thiooxidans), and moderately thermophilic sulfur-oxidizing bacteria Acidianus brierleyi, were cryopreserved in liquid nitrogen and their ferrous ion- or sulfur-oxidizing activities were investigated and compared with the original ones. The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation. Glycerin was used as cryoprotective agent. In conclusion, liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.
Key words: A. ferrooxidans; A. thiooxidans; Acidianus brierleyi; cryopreservation; liquid nitrogen; growth activities
