中国有色金属学报(英文版)
Transactions of Nonferrous Metals Society of China
| Vol. 33 No. 10 October 2023 |
（1. Health Management Center, The Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. Department of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha 410013, China;
3. Department of Urology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;
4. Clinical Class 6 of Grade 2019, Changsha Medical College, Changsha 410219, China）
Abstract:The interaction between terbium-doped arginine functionalized hydroxyapatite nanoparticles (Arg@HAPTb NPs) and cells, and the hindered endocytosis mechanisms of human umbilical vein endothelial cells (HUVECs) were studied by observing the cell uptake of Arg@HAPTb NPs with laser scanning confocal microscopy. Average fluorescence intensity of cells after uptaking different concentrations of NPs was determined by flow cytometer. The results indicate that internalized Arg@HAPTb NPs mainly exist in the cytoplasm of HUVECs, and most of them distribute around the cell nuclei. The entrapment of Arg@HAPTb NPs shows time-dependent and concentration- dependent manners with optimal time of 4 h and concentration of 50 mg/L. Arginine modification significantly improves the uptake efficiency of HAPTb NPs. The entry of Arg@HAPTb NPs into HUVECs is mainly through concave protein-mediated endocytosis, an energy-dependent active transport process.
Key words: hydroxyapatite nanoparticles; transmembrane transport; arginine modification; endocytosis
