Transactions of Nonferrous Metals Society of China The Chinese Journal of Nonferrous Metals

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中国有色金属学报

ZHONGGUO YOUSEJINSHU XUEBAO

第14卷    第2期    总第59期    2004年2月

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文章编号:1004-0609(2004)02-0280-06
硅酸盐细菌的选育及铝土矿细菌脱硅效果
钮因健, 邱冠周, 周吉奎, 覃文庆

(中南大学 资源加工与生物工程学院, 长沙 410083)

摘 要: 为了找、到能从高硅含量铝土矿中脱硅的细菌,利用无氮铝硅酸盐矿物培养基对菜园土、 生物钾肥、 铝土矿等材料进行了硅酸盐细菌的分离, 筛选到一株编号为GSY—5↑#的产荚膜芽孢杆菌。 通过对该细菌的形态、 生理生化等表型特征以及固体、液体培养条件研究, 发现该细菌菌体大小为(1~1.2)μm×(4~7)μm, 荚膜呈椭圆形, 大小为(5~15)μm×(10~30)μm;培养后期产生芽孢, 芽孢呈椭圆形, 大小为(1.5~1.7)μm×(3~4)μm。 显微镜下观察细菌不运动, 没有观察到鞭毛。 该菌生长最适pH为7.3~8.4, 最适生长温度为30℃。 在矿物盐固体培养基上生长良好, 具有较强的固氮能力。 通过比较该菌与胶质芽孢杆菌模式菌株的生理生化特征,可认为GSY—5#菌株是硅酸盐细菌胶质芽孢杆菌。 使用GSY—5#菌株对5种含有不同铝硅酸盐矿物的铝土矿进行了生物脱硅研究, 浸出条件为pH 7.2, 30℃, 200r/min, 矿浆含量5%, 浸出7d。 5种矿样的A/S分别从4.58, 6.74, 6.03, 5.09, 2.93提高到5.88, 8.45, 8.55, 6.79, 13.54, 表明该株硅酸盐细菌具有一定的脱硅能力。 

 

关键字: 硅酸盐细菌; 胶质芽孢杆菌; 筛选; 模式菌株; 表型特征; 铝土矿; 脱硅

Screening of silicate bacteria and bioleaching silicon from bauxite
NIU Yin-jian, QIU Guan-zhou, ZHOU Ji-kui, QIN Wen-qing

School of Resources Processing and Bioengineering,
Central South University, Changsha 410083, China

Abstract: In order to obtain some strains of bacterium which may liberate silicate from bauxite with a high Si content, some strains of bacterium were isolated from materials such as garden soil, biological fertilizer and bauxite mineral using nitrogen media. The phenotype characteristics such as morphology, physiology and biochemical tests show that GSY—5# bacteria belong to Bacillus sp because they are rod shaped and can produce oval endospore. The sizes of bacteria and endospore are (1-1.2)μm×(4-7)μm and (1.5-1.7)μm×(3-4)μm, respectively. The cultivating conditions of GSY—5# on solid media and in liquid media were identified. The results show that GSY—5# grows best within pH range of 7.3-8.4, and the optimum temperature for the growth is 30℃.The microorganism can fix nitrogen because it grows very well on solid nitrogen-free mineral media and show apparent capsule. It can produce a rich variety of exo-polysacchoride in liquid nitrogen-free media. In comparison of characteristics of GSY—5# bacteria with those of mold strain of Bacillus mucilaginosus, GSY—5# bacteria are a strain of Bacillus mucilaginosus. In this experiment five bauxite samples containing aluminosilicates as impurities were leached with silicate bacteria GSY—5# at pH 7.2, 30℃, 200r/min, for 7 days, and the ratio of Al2O3 to SiO2(A/S) in samples is increased from 4.58 to 5.88, from 6.74 to 8.45, from 6.03 to 8.55, from 5.09 to 6.79 and from 2.93 to 13.54, respectively. The leaching results indicate that leaching with silicate bacteria is useful in the improvement of low-grade high-SiO2 bauxite.

 

Key words: silicate bacteria; bacillus mucilaginosus; screening; mold strain; phenotype characteristics; bauxite

ISSN 1004-0609
CN 43-1238/TG
CODEN: ZYJXFK

ISSN 1003-6326
CN 43-1239/TG
CODEN: TNMCEW

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